9ES2
ATP-bound human mitochondrial Hsp60 double-ring complex
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.44 |
| Number of Particle | 1525128 |
| Software | cryoSPARC,Coot,EPU,UCSF Chimera,PHENIX |
| Symmetry | D7 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
