9ER3
Cyanide dihydratase from Bacillus pumilus C1 E155R variant with altered helical twist.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.8 |
| Number of Particle | 2387974 |
| Software | RELION,PHENIX,UCSF ChimeraX,Coot,EPU |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
