9EQM
Crystal structure of pVHL:EloB:EloC in complex with MP-1-21
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-27 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.670, 60.770, 101.260 |
| Unit cell angles | 90.00, 102.50, 90.00 |
Refinement procedure
| Resolution | 46.420 - 2.190 |
| R-factor | 0.2531 |
| Rwork | 0.253 |
| R-free | 0.26360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.100 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.420 | 2.230 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Number of reflections | 22653 | 1121 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.999 | 0.349 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.285 M sodium acetate, 0.1M Bis-Tris pH 5.5, and 5.9% PEG3350 |
