9EM6

OPR3 variant Y364P in its dimeric form obtained without ammonium sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-08
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.87
Spacegroup name	P 1 21 1
Unit cell lengths	58.005, 90.053, 81.241
Unit cell angles	90.00, 109.13, 90.00

Refinement procedure

Resolution	46.810 - 1.720
R-factor	0.1815
Rwork	0.180
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.884
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.810	1.740
High resolution limit [Å]	1.720	1.720
Rpim	0.036	0.980
Number of reflections	81190	1595
<I/σ(I)>	10.14
Completeness [%]	96.9
Redundancy	6.6
CC(1/2)	0.990	0.290

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M Tris-HCl (7.5), 50 mM sodium tartrate, 8-16% PEG8000