9E6E

Yeast Fzf1 Zn-fingers 1-3 bound to YHB1 26 bp DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2002-08-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	61.905, 66.265, 174.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.370 - 2.950
R-factor	0.2198
Rwork	0.218
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.867
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.370	3.140
High resolution limit [Å]	2.950	2.950
Rmerge	0.131	0.932
Rmeas	0.143	1.010
Rpim	0.054	0.386
Number of reflections	15710	2485
<I/σ(I)>	9.6	2
Completeness [%]	100.0	100
Redundancy	6.6	6.7
CC(1/2)	0.997	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	100	0.2 M ammonium acetate, 0.1 M bis-Tris pH 5.5, and 25% w/v PEG 3350