9E2U
Crystal structure of DDB1-CRBN-ALV1 complex bound to triple ZnF of Helios (IKZF2 ZF1-3)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 208.590, 279.059, 286.298 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.330 - 4.110 |
| R-factor | 0.2376 |
| Rwork | 0.235 |
| R-free | 0.28890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.494 |
| Data reduction software | RAPD |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 168.590 | 4.180 |
| High resolution limit [Å] | 4.110 | 4.110 |
| Rmerge | 0.146 | 2.569 |
| Rmeas | 0.165 | 2.878 |
| Rpim | 0.074 | 1.282 |
| Number of reflections | 130289 | 6391 |
| <I/σ(I)> | 9.4 | 0.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 9.3 | 9.8 |
| CC(1/2) | 0.999 | 0.370 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20.455% PEG 3350, 0.214 M Li3 Cit |
