9DZO
Structure of ALAS bound to succinyl-CoA from S. cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920105 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.595, 114.241, 119.307 |
| Unit cell angles | 116.40, 97.51, 91.84 |
Refinement procedure
| Resolution | 48.769 - 2.691 |
| Rwork | 0.180 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.393 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0431 (refmacat 0.4.105)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.770 | 2.840 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmeas | 0.235 | 1.151 |
| Number of reflections | 77425 | 12443 |
| <I/σ(I)> | 5.3 | 1.22 |
| Completeness [%] | 98.5 | 98 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.978 | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.15 M magnesium chloride, 0.1 M HEPES, pH 7.5, 22.5% v/v PEG 3350 |
