Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.534, 98.971, 58.825 |
| Unit cell angles | 90.00, 106.60, 90.00 |
Refinement procedure
| Resolution | 35.930 - 2.200 |
| R-factor | 0.2044 |
| Rwork | 0.203 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.602 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.930 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 30464 | 3025 |
| <I/σ(I)> | 9.69 | |
| Completeness [%] | 99.7 | 99.74 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.995 | 0.839 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 296 | 20% PEG 6000, 0.1M MES pH 6.0, 0.2M NaCl |
