9DS2
Crystal structure of 346-54 Fab in complex with H1 HA from A/California/04/2009(H1N1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0337 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 161.816, 313.101, 325.522 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.820 - 3.290 |
| R-factor | 0.2461 |
| Rwork | 0.244 |
| R-free | 0.27940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.486 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21rc1_5127: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.820 | 3.360 |
| High resolution limit [Å] | 3.290 | 3.300 |
| Rmerge | 0.250 | 1.780 |
| Rpim | 0.087 | 0.650 |
| Number of reflections | 118176 | 117512 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 94.0 | |
| Redundancy | 9 | |
| CC(1/2) | 1.000 | 0.450 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium acetate, pH 6.2, 20% PEG3350 |
