9DRW
Myocilin OLF mutant A427T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2023-11-02 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.930, 47.810, 149.990 |
| Unit cell angles | 90.00, 107.71, 90.00 |
Refinement procedure
| Resolution | 58.620 - 1.450 |
| R-factor | 0.1462 |
| Rwork | 0.146 |
| R-free | 0.18820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.806 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.620 | 1.502 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.064 | 0.538 |
| Rmeas | 0.070 | 0.593 |
| Rpim | 0.027 | 0.241 |
| Number of reflections | 160850 | 12829 |
| <I/σ(I)> | 16.18 | 4.45 |
| Completeness [%] | 95.1 | 76.52 |
| Redundancy | 6.7 | 5.7 |
| CC(1/2) | 0.999 | 0.912 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289 | 10% PEG8000, 0.05M magnesium chloride |
