9DR1
E. coli RNA polymerase consensus volume with a bound fluoride riboswitch in the ligand-bound state
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.7 |
Number of Particle | 419770 |
Software | cryoSPARC,PHENIX,Leginon,UCSF ChimeraX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |