9DPP
BMP-9 Wild-Type Dimer in Acidic pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 70.940, 70.940, 145.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.470 - 2.120 |
| R-factor | 0.2217 |
| Rwork | 0.220 |
| R-free | 0.25770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.557 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.470 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.158 | 2.588 |
| Rmeas | 0.164 | 2.715 |
| Rpim | 0.045 | 0.810 |
| Number of reflections | 10969 | 857 |
| <I/σ(I)> | 14 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.6 | 20.9 |
| CC(1/2) | 0.999 | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 289.15 | 1M NaCl, 3.5% PEG8K, 0.3M Citrate pH 3.5 |
