9DP4
APE1 N174Q Product Complex with Abasic DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-12 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 |
| Unit cell lengths | 153.423, 153.423, 45.373 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.520 - 2.250 |
| R-factor | 0.2208 |
| Rwork | 0.215 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.816 |
| Data reduction software | HKL-3000 (705c) |
| Data scaling software | HKL-3000 (705c) |
| Phasing software | PHENIX (1.19.2-4158-000) |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.520 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmeas | 0.100 | |
| Number of reflections | 63992 | 2206 |
| <I/σ(I)> | 13.74 | 0.84 |
| Completeness [%] | 98.5 | 86.7 |
| Redundancy | 5.6 | 2.4 |
| CC(1/2) | 0.616 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM HEPES free acid, 200 mM ammonium acetate, 25% PEG3350 |
