9DP1

APE1 N174A Substrate Complex with Abasic DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2021-10-08
Detector	RDI CMOS_8M
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	43.943, 60.498, 73.075
Unit cell angles	81.81, 75.60, 88.73

Refinement procedure

Resolution	25.060 - 2.290
R-factor	0.2312
Rwork	0.230
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.705
Data reduction software	XDS
Data scaling software	HKL-3000 (705c)
Phasing software	PHENIX (1.19.2-4158-000)
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.060	2.340
High resolution limit [Å]	2.290	2.290
Rmeas	0.133	0.215
Number of reflections	29791	1460
<I/σ(I)>	7.6	4.39
Completeness [%]	96.6	97.3
Redundancy	2.5	2.6
CC(1/2)		0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	100 mM sodium citrate, pH 5.0, 200 mM magnesium chloride, 16-21% PEG20000