9DNQ

Structure of UBR2-RYF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2022-11-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97648
Spacegroup name	P 1 21 1
Unit cell lengths	25.187, 50.871, 45.956
Unit cell angles	90.00, 89.98, 90.00

Refinement procedure

Resolution	45.960 - 1.220
R-factor	0.1398
Rwork	0.139
R-free	0.15020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.978
Data reduction software	DIALS
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.240
High resolution limit [Å]	1.220	3.310	1.220
Rmerge	0.087	0.038	0.178
Rmeas	0.098	0.043	0.208
Rpim	0.046	0.020	0.105
Total number of observations	141414
Number of reflections	33994	1742	1585
<I/σ(I)>	16
Completeness [%]	98.1	97.2	92.4
Redundancy	4.2	4.4	3.5
CC(1/2)	0.995	0.998	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	The stock concentration of UBR2UBR for co-crystallization is 6.2 mg/ml. For UBR2UBR-RYF the ligand to protein molar ratio is 1:2 and the reservoirs contain 0.1 M Bis-Tris pH 6.3 with 26% PEG 3350.