9DNN
Insulin receptor in complex with both insulin and de novo designed site-2 binder "S2B".
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | TFS GLACIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 6.1 |
Number of Particle | 977361 |
Software | RELION,PHENIX,SerialEM,Gctf,Coot |
Symmetry | C2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |