Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.18057 |
| Spacegroup name | I 4 |
| Unit cell lengths | 89.749, 89.749, 85.963 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.590 - 1.600 |
| R-factor | 0.1668 |
| Rwork | 0.166 |
| R-free | 0.19040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.887 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2-3874_final) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.870 | 1.210 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.054 | 4.115 |
| Rmeas | 0.059 | 5.722 |
| Rpim | 0.023 | 3.963 |
| Total number of observations | 573257 | |
| Number of reflections | 95597 | 2306 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 86.9 | |
| Redundancy | 6 | 1.4 |
| CC(1/2) | 1.000 | 0.010 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris pH 8.5, 0.2 M sodium citrate, & 30% PEG400 |
