9DBT

Crystal structure of human astrovirus 1 capsid spike bound to human neonatal Fc receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	295
Detector technology	PIXEL
Collection date	2023-11-11
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.977
Spacegroup name	P 2 21 21
Unit cell lengths	87.000, 152.999, 255.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.030 - 3.400
R-factor	0.2105
Rwork	0.209
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.287
Data reduction software	DIALS (v3-16-1)
Data scaling software	DIALS (v3-16-1)
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	255.360	3.460
High resolution limit [Å]	3.400	3.400
Rmerge	0.169	1.094
Rmeas	0.174	1.123
Rpim	0.039	0.249
Number of reflections	47857	2384
<I/σ(I)>	9.2	0.9
Completeness [%]	100.0	100
Redundancy	19.7	20.2
CC(1/2)	0.998	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	295.15	Sodium chloride, Ammonium sulfate, BIS-TRIS