9D8Q
Crystal structure of Estrogen Receptor alpha from Melanotaenia fluviatilis bound to estradiol and human SRC2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-11-21 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 167.184, 167.184, 167.184 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.680 - 2.751 |
| Rwork | 0.226 |
| R-free | 0.26110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.260 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.158 | 3.586 |
| Rmeas | 0.160 | 3.629 |
| Rpim | 0.025 | 0.555 |
| Total number of observations | 428751 | 64840 |
| Number of reflections | 10699 | 1533 |
| <I/σ(I)> | 25.4 | 1.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 40.1 | 42.3 |
| CC(1/2) | 1.000 | 0.689 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 9-10% Tacsimate pH 7.0, 22% PEG3000 |
