9D7T

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Api137 antimicrobial peptide, mRNA, A-site release factor 1, and deacylated P-site and E-site tRNAphe at 2.70A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-25
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.600, 450.810, 624.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	152.870 - 2.700
R-factor	0.2298
Rwork	0.227
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.837
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	152.870	152.870	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.205	0.032	2.024
Rmeas	0.225	0.035	2.233
Total number of observations	9461928
Number of reflections	1580937	18597	116065
<I/σ(I)>	8.51	35.72	0.89
Completeness [%]	99.1	98.5	98.8
Redundancy	5.985	6.083	5.537
CC(1/2)	0.994	0.999	0.134

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME