9D5K
Human Adenosine Deaminase Acting on dsRNA (ADAR2-RD) bound to dsRNA containing an expanded cytidine analog at the -1 position of the guide strand
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-25 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.775, 63.112, 141.064 |
| Unit cell angles | 90.00, 118.54, 90.00 |
Refinement procedure
| Resolution | 49.460 - 2.697 |
| R-factor | 0.2234 |
| Rwork | 0.221 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.635 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.962 | 2.879 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.070 | 0.623 |
| Rmeas | 0.083 | 0.739 |
| Rpim | 0.044 | 0.392 |
| Number of reflections | 27371 | 1369 |
| <I/σ(I)> | 10.3 | 2.1 |
| Completeness [%] | 92.0 | 64.4 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.897 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 50 mM MOPS pH 7.0, 100 mM NaCl, 10% PEG 4000 |
