9D0J

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with BT-33, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.260, 449.690, 619.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	121.860 - 2.500
R-factor	0.2199
Rwork	0.218
R-free	0.26040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.847
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	121.860	121.860	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.159	0.024	1.859
Rmeas	0.176	0.027	2.070
Total number of observations	9964329
Number of reflections	1973154	23103	145640
<I/σ(I)>	9.58	41.93	0.94
Completeness [%]	99.4	98.7	99.6
Redundancy	5.05	5.297	4.997
CC(1/2)	0.995	0.998	0.178

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME