9D0I

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with Se-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.45A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	209.790, 449.980, 627.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	123.690 - 2.450
R-factor	0.2198
Rwork	0.217
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.008
RMSD bond angle	1.397
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	123.690	123.690	2.510
High resolution limit [Å]	2.450	10.960	2.450
Rmerge	0.167	0.047	2.341
Rmeas	0.182	0.051	2.552
Total number of observations	13829187
Number of reflections	2135930	24901	157522
<I/σ(I)>	8.91	34.02	0.88
Completeness [%]	99.8	98.9	99.9
Redundancy	6.475	6.946	6.233
CC(1/2)	0.995	0.996	0.151

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME