9D0H

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with C-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	210.310, 450.930, 625.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	125.540 - 2.500
R-factor	0.2195
Rwork	0.217
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.010
RMSD bond angle	1.573
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	125.540	125.540	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.167	0.065	1.940
Rmeas	0.185	0.072	2.144
Total number of observations	10461729
Number of reflections	1993213	23302	143230
<I/σ(I)>	7.7	22.08	0.94
Completeness [%]	98.6	97.9	96.1
Redundancy	5.249	5.36	5.186
CC(1/2)	0.991	0.992	0.183

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME