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9D0H

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with C-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyPIXEL
Collection date2022-11-11
DetectorDECTRIS EIGER2 X 16M
Wavelength(s)0.97918
Spacegroup nameP 21 21 21
Unit cell lengths210.310, 450.930, 625.880
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution125.540 - 2.500
R-factor0.2195
Rwork0.217
R-free0.26640
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)6xhw
RMSD bond length0.010
RMSD bond angle1.573
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX (1.8.2)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]125.540125.5402.560
High resolution limit [Å]2.50011.1802.500
Rmerge0.1670.0651.940
Rmeas0.1850.0722.144
Total number of observations10461729
Number of reflections199321323302143230
<I/σ(I)>7.722.080.94
Completeness [%]98.697.996.1
Redundancy5.2495.365.186
CC(1/2)0.9910.9920.183
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION7.62920.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME

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