9D0H
Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with C-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-11 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 210.310, 450.930, 625.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 125.540 - 2.500 |
R-factor | 0.2195 |
Rwork | 0.217 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xhw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.573 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 125.540 | 125.540 | 2.560 |
High resolution limit [Å] | 2.500 | 11.180 | 2.500 |
Rmerge | 0.167 | 0.065 | 1.940 |
Rmeas | 0.185 | 0.072 | 2.144 |
Total number of observations | 10461729 | ||
Number of reflections | 1993213 | 23302 | 143230 |
<I/σ(I)> | 7.7 | 22.08 | 0.94 |
Completeness [%] | 98.6 | 97.9 | 96.1 |
Redundancy | 5.249 | 5.36 | 5.186 |
CC(1/2) | 0.991 | 0.992 | 0.183 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |