9D0G

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with O-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	209.400, 449.820, 621.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	147.220 - 2.500
R-factor	0.219
Rwork	0.217
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.009
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	147.220	147.220	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.160	0.042	1.989
Rmeas	0.176	0.046	2.172
Total number of observations	12001413
Number of reflections	1984527	23131	146559
<I/σ(I)>	8.73	29.19	0.9
Completeness [%]	99.6	98.6	99.9
Redundancy	6.047	6.08	6.136
CC(1/2)	0.995	0.996	0.192

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME