Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 66.576, 71.301, 177.507 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.930 - 2.010 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.23730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.624 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.660 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.075 | 1.237 |
| Rmeas | 0.084 | 1.395 |
| Rpim | 0.038 | 0.635 |
| Total number of observations | 261353 | 18601 |
| Number of reflections | 57245 | 4012 |
| <I/σ(I)> | 9.4 | 1.1 |
| Completeness [%] | 99.6 | |
| Redundancy | 4.6 | 4.6 |
| CC(1/2) | 0.993 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 281 | 0.2 M Sodium Acetate 10% PEG 8000 (w/v) 0.1 M trisodium citrate-citric acid pH 5.4 |
