Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-03 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.527, 70.589, 119.831 |
| Unit cell angles | 90.00, 94.23, 90.00 |
Refinement procedure
| Resolution | 119.500 - 2.097 |
| R-factor | 0.3025 |
| Rwork | 0.301 |
| R-free | 0.32080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.850 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8 (10-JUL-2024)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.500 | 2.200 |
| High resolution limit [Å] | 2.097 | 2.100 |
| Number of reflections | 41982 | 2100 |
| <I/σ(I)> | 5.8 | 1.5 |
| Completeness [%] | 88.9 | 80.5 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.996 | 0.673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 0.1 M Bis-Tris pH 5.5, 0.2 M magnesium chloride, 25% (w/v) PEG 3,350 |
