9CSN

Crystal structure of human ribonuclease 7 (RNase 7) in complex with 5'-adenosine monophosphate (5'-AMP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.1808
Spacegroup name	P 1 21 1
Unit cell lengths	43.110, 48.170, 62.900
Unit cell angles	90.00, 97.28, 90.00

Refinement procedure

Resolution	42.760 - 2.070
R-factor	0.2215
Rwork	0.217
R-free	0.26240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.696
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX ((1.19.1_4122: 000))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.770	42.770	1.970
High resolution limit [Å]	1.910	4.250	1.910
Rmerge	0.157	0.044	5.029
Rmeas	0.170	0.047	5.532
Rpim	0.066	0.018	2.271
Number of reflections	19968	1884	1779
<I/σ(I)>	8.3	31.9	0.3
Completeness [%]	99.6	99.9	98.6
Redundancy	6.6	6.4	5.8
CC(1/2)	0.998	0.999	0.242

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1M MIB (malonic acid:imidazole:boric acid = 2:3:3 molar ratio), pH 7.0, 25% w/v PEG 1.500