9CSM

Crystal structure of human ribonuclease 7 (RNase 7, HsR7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.18082
Spacegroup name	P 1 21 1
Unit cell lengths	25.221, 62.250, 38.276
Unit cell angles	90.00, 99.20, 90.00

Refinement procedure

Resolution	37.780 - 1.770
R-factor	0.1802
Rwork	0.177
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.618
Data reduction software	XDS
Data scaling software	DIALS
Refinement software	PHENIX ((1.19.1_4122: 000))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.790	37.790	1.850
High resolution limit [Å]	1.770	3.540	1.770
Rmerge	0.621	0.287	2.073
Rmeas	0.634	0.293	2.114
Rpim	0.125	0.060	0.408
Number of reflections	11450	1455	1428
<I/σ(I)>	13.7	30.7	3.4
Completeness [%]	100.0	99.9	100
Redundancy	24.6	23.1	25.3
CC(1/2)	0.994	0.992	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	0.01M Zinc chloride, 0.1M MES, pH 6.0, 20% PEG6000