9CPL
The ubiquitin-associated domain of human thirty-eight negative kinase 1, fused to the 2TEL crystallization chaperone via a 2-glycine linker
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-08-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 65 |
Unit cell lengths | 105.725, 105.725, 50.917 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.780 - 2.400 |
R-factor | 0.2313 |
Rwork | 0.229 |
R-free | 0.26340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.480 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.780 | 2.400 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.103 | 3.597 |
Rmeas | 0.108 | 3.786 |
Rpim | 0.034 | 1.173 |
Number of reflections | 12862 | 1150 |
<I/σ(I)> | 10.88 | 0.62 |
Completeness [%] | 99.1 | 92 |
Redundancy | 10.2 | 10.2 |
CC(1/2) | 0.999 | 0.449 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 100mM BisTris, pH 6.0, 960mM Mg-formate |