9CKO

Human G protein-coupled receptor kinase 5 wild-type in complex with sangivamycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	277
Detector technology	PIXEL
Collection date	2023-02-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03317
Spacegroup name	P 41 21 2
Unit cell lengths	140.259, 140.259, 78.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.450 - 2.800
R-factor	0.2432
Rwork	0.240
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.573
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.730	2.800
High resolution limit [Å]	2.670	2.730
Rmerge	0.247
Number of reflections	22966	2242
<I/σ(I)>	7.7
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)		0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	200mM potassium citrate tribasic, 20% polyethylene glycol 3350