9CCV
Crystal structure of human respiratory syncytial virus NS1 bound to human MED25 ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-10 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 104.953, 104.953, 63.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.230 - 2.530 |
| R-factor | 0.24343 |
| Rwork | 0.242 |
| R-free | 0.26211 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.805 |
| Data reduction software | HKL-3000 (718) |
| Data scaling software | HKL-3000 (718) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.073 | 0.048 |
| Number of reflections | 12815 | 622 |
| <I/σ(I)> | 54 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25 | |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.15 M ammonium tartrate, 0.1 M MES pH 5.6, 12% PEG 3350 |
