9CCV

Crystal structure of human respiratory syncytial virus NS1 bound to human MED25 ACID

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-10
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.00002
Spacegroup name	P 43 21 2
Unit cell lengths	104.953, 104.953, 63.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.230 - 2.530
R-factor	0.24343
Rwork	0.242
R-free	0.26211
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.805
Data reduction software	HKL-3000 (718)
Data scaling software	HKL-3000 (718)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.073	0.048
Number of reflections	12815	622
<I/σ(I)>	54
Completeness [%]	100.0	100
Redundancy	25
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.15 M ammonium tartrate, 0.1 M MES pH 5.6, 12% PEG 3350