9CBS
Crystal structure of Chaetomium thermophilum Gcn2 HisRS-like domain, catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 126.091, 132.554, 138.961 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.490 - 2.690 |
R-factor | 0.1779 |
Rwork | 0.177 |
R-free | 0.20960 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.701 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.740 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.210 | 1.270 |
Rpim | 0.037 | 0.283 |
Number of reflections | 65096 | 3228 |
<I/σ(I)> | 48.1 | 4 |
Completeness [%] | 98.8 | 93.8 |
Redundancy | 33.2 | 20 |
CC(1/2) | 0.916 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.15 M MgCl2, 0.15 M CaCl2, 100 mM HEPES pH 7.5, 20% v/v PEG 500 MME, and 5% PEG 20000 |