9C1Q
Crystal structure of the WDR domain of human DCAF1 in complex with OICR-39512 compound
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 82.340, 82.340, 233.617 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.220 - 1.800 |
| R-factor | 0.15759 |
| Rwork | 0.157 |
| R-free | 0.19136 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.271 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.063 | 0.025 | 0.950 |
| Rmeas | 0.065 | 0.026 | 0.985 |
| Rpim | 0.018 | 0.007 | 0.258 |
| Number of reflections | 44430 | 2530 | 2169 |
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 99.9 | 99.8 | 99.9 |
| Redundancy | 13.7 | 12 | 14.3 |
| CC(1/2) | 1.000 | 1.000 | 0.871 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG3350, 0.2 M Magnesium formate |
