9C13
XMAP215 TOG5 interaction with GMPCPP tubulin lattice
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.1 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.89 |
| Software | cryoSPARC,EPU,Coot,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
