9BVD

Crystal structure of SRY HMG box bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97931
Spacegroup name	P 1 21 1
Unit cell lengths	59.506, 91.226, 73.756
Unit cell angles	90.00, 106.39, 90.00

Refinement procedure

Resolution	35.380 - 2.480
R-factor	0.2012
Rwork	0.200
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y60
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.090	57.090	2.610
High resolution limit [Å]	2.480	7.830	2.480
Rmerge	0.036	0.031	0.416
Rmeas	0.042	0.038	0.492
Rpim	0.023	0.020	0.260
Total number of observations	91922	2878	13633
Number of reflections	26500	867	3888
<I/σ(I)>	12	24.3	2.5
Completeness [%]	98.2	96.7	99
Redundancy	3.5	3.3	3.5
CC(1/2)	0.998	0.996	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M KCl, 0.1M Mg(OAc)2, 0.05M MES 6.5, 8% PEG 8000