Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99989 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 91.018, 91.018, 121.995 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 78.820 - 2.080 |
| R-factor | 0.19238 |
| Rwork | 0.191 |
| R-free | 0.22795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.739 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.820 | 2.330 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.089 | 0.498 |
| Rmeas | 0.099 | 0.554 |
| Number of reflections | 33919 | 8974 |
| <I/σ(I)> | 13.63 | 3.84 |
| Completeness [%] | 95.1 | 88.4 |
| Redundancy | 5.2 | 5.2 |
| CC(1/2) | 0.998 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.10 M NaAcetat_pH4.8, 2.30 M (NH4)2SO4, 30 % Glycerol, 18% (w/v) PEG 4000, 0.1M Na3Citrate pH=5.75 |
