9BJG

Crystal structure of broadly neutralizing human monoclonal antibody 75B10 in complex with AMA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	93.236, 93.236, 228.015
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.880 - 2.900
R-factor	0.2013
Rwork	0.199
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.587
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.20.1-4487)
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.880	2.980
High resolution limit [Å]	2.900	2.900
Rmerge	0.259	1.594
Rmeas	0.273	1.679
Rpim	0.085	0.522
Number of reflections	26126	1954
<I/σ(I)>	4.5	0.59
Completeness [%]	92.3	77.49
Redundancy	10.1	10
CC(1/2)	0.987	0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291.15	0.2 M calcium acetate, 0.1 M Imidazole, 20% PEG 1000