Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2018-10-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.300, 75.860, 86.870 |
| Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
| Resolution | 34.700 - 2.250 |
| R-factor | 0.2033 |
| Rwork | 0.200 |
| R-free | 0.27110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.545 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.814 | 2.035 |
| High resolution limit [Å] | 2.028 | 2.028 |
| Rmerge | 0.095 | 1.696 |
| Rmeas | 0.113 | 2.184 |
| Rpim | 0.061 | 1.347 |
| Total number of observations | 531 | |
| Number of reflections | 65504 | 214 |
| <I/σ(I)> | 6.7 | 0.6 |
| Completeness [%] | 89.9 | |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 292 | 0.1 M Tris-HCl, pH 8.5, 0.25 M sodium tartrate and 12% PEG 8000 |
