Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95358 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.140, 101.630, 66.230 |
| Unit cell angles | 90.00, 90.94, 90.00 |
Refinement procedure
| Resolution | 55.480 - 1.140 |
| R-factor | 0.1425 |
| Rwork | 0.141 |
| R-free | 0.17720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.170 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.480 | 1.181 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.087 | 1.399 |
| Rpim | 0.035 | 0.676 |
| Number of reflections | 220420 | 17280 |
| <I/σ(I)> | 9.62 | 0.96 |
| Completeness [%] | 95.4 | 74.91 |
| Redundancy | 6.8 | 5 |
| CC(1/2) | 0.999 | 0.363 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 28% PEG 3350, 0.1M Bis Tris, pH 5.5, 150 nM NaCl |
