9BF9
Human LAG-3-HLA-DR1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.714, 148.067, 85.586 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.560 - 3.400 |
| R-factor | 0.2106 |
| Rwork | 0.206 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.473 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.560 | 3.522 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rpim | 0.073 | 0.650 |
| Number of reflections | 32890 | 1716 |
| <I/σ(I)> | 14.52 | 1.33 |
| Completeness [%] | 99.7 | 99.13 |
| Redundancy | 10.3 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 22% PEG 8000, 0.16M Li2SO4, 0.1M HEPES pH 7.6 |
