9BD3
Structure of the MAGEA4 MHD-RAD18 R6BD Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 65 |
Unit cell lengths | 85.210, 85.210, 206.080 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.600 - 2.580 |
R-factor | 0.1929 |
Rwork | 0.191 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.571 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.600 | 2.672 |
High resolution limit [Å] | 2.580 | 2.580 |
Rmerge | 0.065 | 0.818 |
Rpim | 0.028 | 0.361 |
Number of reflections | 26114 | 2606 |
<I/σ(I)> | 19.04 | 3.79 |
Completeness [%] | 98.2 | |
Redundancy | 6.1 | |
CC(1/2) | 0.999 | 0.877 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% (w/v) PEG 8000 100 mM HEPES/ Sodium hydroxide, pH 7.5, 200 mM Ammonium sulfate, 10% (v/v) 2-propanol |