Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.609, 71.609, 309.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.750 - 2.750 |
| R-factor | 0.2483 |
| Rwork | 0.246 |
| R-free | 0.29916 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.975 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.750 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.407 | 3.602 |
| Rmeas | 0.424 | 3.762 |
| Rpim | 0.117 | 1.075 |
| Total number of observations | 37421 | |
| Number of reflections | 22176 | 3134 |
| <I/σ(I)> | 7.7 | 0.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.8 | 11.9 |
| CC(1/2) | 0.991 | 0.517 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 1.3-1.5M Ammonium Sulfate, 0.050 M Tris.HCl(pH8.9), 0.050M Bicine (pH 9) |
