9BA2
Crystal structure of the binary complex of DCAF1 and WDR5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.18061 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.597, 83.185, 158.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.580 - 2.970 |
| R-factor | 0.2314 |
| Rwork | 0.230 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.580 | 3.200 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.021 | 0.216 |
| Rmeas | 0.029 | 0.305 |
| Rpim | 0.021 | 0.216 |
| Number of reflections | 13571 | 2648 |
| <I/σ(I)> | 23.01 | |
| Completeness [%] | 100.0 | 99.96 |
| Redundancy | 2 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M imidazole, pH 7.4, 20% w/v polyethylene glycol 4,000 |
