9B4T

Crystal structure of the MRAS-p110alpha complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	58.935, 105.936, 114.493
Unit cell angles	90.00, 97.50, 90.00

Refinement procedure

Resolution	50.030 - 2.750
R-factor	0.2158
Rwork	0.213
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.406
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.030	50.030	2.920
High resolution limit [Å]	2.750	8.160	2.750
Rmerge	0.078	0.034	1.119
Rmeas	0.084	0.037	1.205
Number of reflections	35877	1404	5713
<I/σ(I)>	13.48
Completeness [%]	98.7
Redundancy	7
CC(1/2)	0.999	0.999	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M (Tris/Bicine) 1.2%w/v cholic acid mix, 50%v/v (40%v/v ethylene glycol, 20%w/v PEG 8000)