9B3N

Human Notch-1 EGFs 20-24

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 1
Unit cell lengths	28.030, 35.080, 63.890
Unit cell angles	99.71, 92.35, 96.86

Refinement procedure

Resolution	62.850 - 1.500
R-factor	0.1767
Rwork	0.175
R-free	0.19790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.464
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.900	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.056	0.483
Number of reflections	36167	2764
<I/σ(I)>	7.7	1.7
Completeness [%]	95.2
Redundancy	2.6
CC(1/2)	0.996	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Imidazole Malate pH 6 8% (w/v) PEG4K