9B0Z
Structure of Optineurin bound to HOIP NZF1 domain and M1-linked diubiquitin, crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 247.573, 72.313, 56.345 |
| Unit cell angles | 90.00, 95.07, 90.00 |
Refinement procedure
| Resolution | 44.180 - 2.410 |
| R-factor | 0.2231 |
| Rwork | 0.221 |
| R-free | 0.26950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.813 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.440 | 2.830 |
| High resolution limit [Å] | 2.400 | 2.700 |
| Rmerge | 0.087 | 0.630 |
| Rmeas | 0.106 | 0.769 |
| Rpim | 0.059 | 0.435 |
| Total number of observations | 10863 | |
| Number of reflections | 27383 | 3582 |
| <I/σ(I)> | 7.6 | 1.2 |
| Completeness [%] | 99.6 | |
| Redundancy | 3.1 | 3 |
| CC(1/2) | 0.989 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 20% PEG 2K MME, 0.2 M TAO and 0.1 M Tris pH 8.5. Cryoprotected in mother liquor containing 20% glycerol |
