9B0B

Structure of Optineurin bound to HOIP NZF1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96859
Spacegroup name	P 21 21 21
Unit cell lengths	49.755, 92.684, 149.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.265 - 1.700
R-factor	0.1974
Rwork	0.196
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.527
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.820	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.081	0.830
Rmeas	0.088	0.904
Rpim	0.035	0.355
Total number of observations	493784	25734
Number of reflections	77197	4026
<I/σ(I)>	12.1	1.9
Completeness [%]	100.0
Redundancy	6.4	6.4
CC(1/2)	0.999	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	3:1 (protein:mother liquor: 10% PEG 8K, 20% ethylene glycol, 0.1 M Tris/Bicine pH 8.5, 0.03 M MgCl2 and 0.03 M CaCl2. cryoprotected in mother liquor containing 10% glycerol