9AVR

Human eIF4A-1 in complex with AMP-PNP, RNA, and the inhibitor silvestrol

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.953
Spacegroup name	P 21 21 21
Unit cell lengths	58.480, 84.295, 88.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.210 - 1.910
R-factor	0.1519
Rwork	0.147
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.466
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18_3861)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	1.900	2.000
Number of reflections	33958	752
<I/σ(I)>	0.3221
Completeness [%]	98.0
Redundancy	6.5
CC(1/2)	0.956	0.456

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2 M sodium iodide, 20% PEG3350