9ZUZ
Translation Initiation Factor eIF-2B complex with the Isohexide Diamine GSK735
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 108.529, 153.968, 138.217 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.130 - 2.250 |
| R-factor | 0.1817 |
| Rwork | 0.180 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.512 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.130 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.081 | |
| Number of reflections | 406064 | 1977 |
| <I/σ(I)> | 24.7 | |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 8 | |
| CC(1/2) | 0.505 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M Proline, 0.1 M hepes pH 7.5, 8.6 % Peg3350 |
